Окиснювальний баланс у фолікулярній рідині яєчників: кореляція з гормональним профілем та відповіддю яєчників у циклах екстракорпорального запліднення

Анотація

The objective: to evaluate the oxidation-reduction potential (ORP) of follicular fluid (FF) in ovaries as a biomarker of the intrafollicular microenvironment and its association with oocyte maturation and in vitro fertilization (IVF) outcomes.
Materials and methods. A retrospective analysis of 212 IVF cycles during 2020–2023 was conducted. ORP in FF was measured using MiOXSYS. The concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol, anti-Müllerian hormone and prolactin were measured in blood serum. Correlations between ORP and gonadotropin levels, stimulation parameters, and oocyte outcomes were assessed.
Results. The mean ORP value in FF was 86.42 ± 23.23 mV, with wide interindividual variations. Weak but statistically significant positive correlations were observed between ORP and baseline FSH (ρ = 0.166, p = 0.016) and LH levels (ρ = 0.148, p = 0.032). Patients with basal FSH ≥ 8 IU/L exhibited higher ORP levels compared to women with FSH concentration < 8 IU/L (median 93.26 mV vs 81.70 mV, p = 0.041). However, ORP values did not differ significantly between groups stratified by total FSH dose, estradiol levels on chorionic gonadotropin day, or total number of retrieved oocytes. Notably, patients achieving ≥ 12 mature oocytes had a significantly higher oocyte maturation rate (86.19% vs 77.78%, p < 0.001), although their median ORP values were not significantly different (84.17 mV vs 89.68 mV, p = 0.849).
Conclusions. ORP in FF reflects subtle endocrine influences but was not directly predictive of oocyte number or maturity. Stable ORP values together with optimal stimulation (≥ 12 mature oocytes) were associated with better oocyte maturation, suggesting ORP as a potential marker of follicular health in IVF.